Aquaculture 512, 734249 (2019). The complete dataset of raw data was deposited into NCBI`s Sequence Read Archive under the accession number PRJNA628161. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in CAS  10, 302–309 (2012). Int. In this study, we set the Smith-Waterman hybridization alignments match score higher than 140 and the minimum free energy of the duplex structure less … Rev. Miranda NG 0.95.13 Deutsch: Miranda NG ist ein kostenloser Multi-Messenger für Facebook, Skype, ICQ und Co. The DE miRNAs are identified, which are promising to regulate the shell color polymorphism. Mar. A total of 114 miRNAs were identified to be with > 1 TPM in each library. A total of eight DE miRNAs were selected to determine their expression levels in four samples by qRT-PCR. Langmead, B. Inhibitory effect of microRNA-34a on retinal pigment epithelial cell proliferation and migration. Both ECM-receptor interaction and Notch signaling pathway were significantly enriched in WS vs NS comparison (Fig. By submitting a comment you agree to abide by our Terms and Community Guidelines. Google Scholar. Of them, lgi-miR-317 and its targets peroxidase and lncRNA TCONS_00951105 are implicated in acting as the competing endogenous RNA to regulate melanogenesis. BMC Genomics 15, 1122–1122 (2014). While the solid pigmentation represented one color in a shell, which could be black, white or yellow. View the profiles of people named Mirna Miranda. After alignment and additional sequence analysis, a total of 144 candidate miRNAs from mantle libraries were identified, including 53 known miRNAs and 91 novel miRNAs (Supplementary Table S2). And lgi-miR-317 might act as a ceRNA to de-repress the expression of peroxidase (LOC_105324712, XM_011423866) and its potential cis-acting lincRNA TCONS_00951105 to regulate melanin synthesis, by binding to these two transcript. CAS  The miRNA-mRNA networks for transcription and phosphorylation were analyzed. Wienholds, E. et al. Google Scholar. Biotechnol. Proteom. Mapped small RNA tags were used to look for known miRNA from miRBase 21.0 (ftp://mirbase.org/pub/mirbase/). Values are shown as mean ± SD (n = 3), *, P < 0.05, **, P < 0.01. Der frühere SourceForge-Projektname lautete „Miranda-ICQ“, da das Projekt inzwischen aber neben ICQ auch viele weitere Protokolle unterstützt, wurde der Name 2003 geändert. 92, 1039–1058 (2017). This study was supported by Grants from National Natural Science Foundation of China (31972789 and 31772843), Shandong Province (2017LZGC009), and the Ocean University of China-Auburn University Joint Research Center for Aquaculture and Environmental Science. These findings will further be used to improve artificial selection efficiency and contribute to the genetic improvements of the oyster aquaculture. Sci. By integrating miRNA and mRNA expression profiles, a total of 870 genes were predicted as targets of differentially expressed miRNAs, mainly involving in biomineralization and pigmentation through functional enrichment. A total of 53 conserved miRNAs in all four mantle libraries were grouped into 38 miRNA families according to the new miRNA family classifications in Rfam. In this study, we investigated miRNAs profiles in mantle of C. gigas characterized by shell color (WS, GS, BS, NS) using high-throughput sequencing. zum Download. 1, the majority of the sRNAs sequenced were 21–23 nt in length, and the most frequent length of sRNA was 22 nt. A total of 144 miRNAs were identified, 47 miRNAs were detected to differentially express. Google Scholar. R Bioconductor, 8, 1–25 (2012). In WS vs NS comparison, 22 up-regulated miRNAs were predicted to negatively regulate 136 down-regulated mRNAs, 8 down-regulated miRNAs were predicted to negatively regulate 80 up-regulated mRNAs (Supplementary Table S4). Library quality was assessed on the Agilent Bioanalyzer 2100 system using DNA High Sensitivity Chips. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Google Scholar. Rong, X., Zhang, Y. K., Zhang, K. J. Zhao, X. L., Yu, H., Kong, L. F., Liu, S. K. & Li, Q. Biol. Gene 666, 32–43 (2018). Software & Apps zum Download, sowie Cloud-Dienste für Windows, Mac, Linux, iPhone, Android. PubMed  PubMed  CAS  Identification of miR-145 as a key regulator of the pigmentary process. PubMed  ADS  In all four libraries, miRNAs with TPM ≥ 1000 were classified as abundant while those with TPM < 1 were classified as rare.